Abstract
Under phosphate deficiency, phosphate re-translocation from older organs to younger organs is one of the important adaptive mechanisms. In the former study, we measured phosphate distribution in shoot which was hydroponically cultured and determined source and sink leaves. Then, we searched potential genes involving phosphate re-translocation in Arabidopsis using microarray analysis of m-RNA isolated from source and sink leaves. Finally we identified some possible genes which changed transcriptional levels between source and sink leaves.
In the present study, we further repeated microarray analysis and confirmed expression levels of genes related to source-sink status with quantitative PCR method. We are now measuring phosphate distribution in T-DNA insertion lines of those genes, and discuss the possible roles of source-sink related genes in phosphate re-translocation.
