Abstract
The role of plant purple acid phosphatases (PAPs) has ever been proposed for the degradation of organophosphates, when plants are under phosphate starvation. However, not only wall regeneration on the surface of tobacco protoplasts was accelerated by the addition of wall-bound tobacco PAP, but also cellulose deposition was enhanced in the transgenic tobacco cells overexpressing the PAP (NtPAP12). Since PAPs are members of a group of nonspecific phoshomonoesterase, containing protein phosphatases, calcineurin (type 2B) and protein phosphatases type 1, the tobacco PAP could probably function as the type of protein phosphatase. We suggest the potential substrates for NtPAP12 in suspension-cultured tobacco cells by screening proteins phosphorylated.
