Abstract
Many cyanobacterial species possess two copies of genes encoding putative transcriptional regulators having an AbrB-type DNA binding domain at their C-termini. In this study, we made the disrupted mutants of sll0359 and sll0822 encoding AbrB-type regulators in Synechocystis sp. PCC 6803. Under low-light conditions, the content of chlorophyll and phycocyanin in sll0359 disruptant was about 70% of that in the wild-type cells. The doubling time of the mutant was 1.75 times longer than that of the wild type. Northern blot analysis revealed that the expression levels of genes encoding subunits of photosystem I and enzymes for pigment biosynthesis were lower in the mutant. Under high-light conditions, growth rate and pigment content were similar between the wild type and the mutant, indicating that the ability of high-light acclimation was not affected by the disruption of sll0359. Characterization of sll0822 disruptant will be also reported.
