Abstract
Japanese cedar (Cryptomeria japonica D. Don) is one of the major forest tree species in Japan, but recently more than 10 % of the population are suffered from its pollinosis. Genetic modification might be a powerful tool to minimize breeding period of time and can attach only our favorable traits. We have succeeded in regenerating Japanese cedar plantlets by somatic embryogenesis via embryogenic tissues derived from immature embryo collected in June to July, and in producing transgenic Japanese cedar by infection of the embryogenic tissues with Agrobacterium which harbouring a binary vector that contain Maize Ubiquitin Promoter -sGFP(S65T) construct.
Eleven weeks after the infection three transgenic lines those which GFP fluorescence can observed were isolated and propagated tissues were transferred to embryo induction media. Regenerated plantlets were obtained finally 7 months after the infection and they were supplied for further analysis to confirm integration of the genes to the genome.
