Abstract
It is difficult to extend the phenotyping of mutants to genome-wide scale because mutants require the suitable condition to exhibit their phenotype. To find as much information as possible, we focused on chlorophyll fluorescence emitted from cyanobacteria. Cyanobacterial photosynthetic system is not separated from other metabolic systems. Therefore chlorophyll fluorescence that is indicator for photosynthesis could reflect the state of other metabolic systems. The kinetics of 303 mutants grown under high and low light conditions was observed. Among them, 203 mutants exhibited distinctly different phenotypes from the wild type. The percentage of unknown gene disruptants that showed phenotype only when grown under high light condition was higher than that of annotated gene disruptants. The result might imply that unknown gene disruptant group contains a high percentage of the mutant whose symptoms of gene disruption might be observed only under specific condition.
