Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 47
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Development of Real-Time imaging system for radioactive tracers in a living plant.
*Hiroki RaiSatomi KannoTomoyuki OhyaKeitaro TanoiTomoko Nakanishi
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Pages 300

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Abstract
We developed 2-dimensional real-time imaging system for beta-ray emitters within a plant to analyze chemical translocation or signal transmission. When a radioactive tracer was applied to a plant, a beta-ray emitted from the plant was converted to light through a scintillator and then, the weak light was detected by a photon-counting camera. The image obtained by this system had a very high sensitivity for 32P, about ten times higher than that of an imaging plate (IP) with similar resolution. It was noted that even 45Ca and 14C, which beta-ray energy was very low was also able to apply for this system. It is also important for precise imaging analysis that the image is quantitative or not. Now we are trying to make this system allows to take quantitative images with 32P and young soybean plants.
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© 2006 by The Japanese Society of Plant Physiologists
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