Abstract
It has previously been shown that desiccation tolerance of cultured cells of M. polymorpha is increased by preculture in a medium with 0.5M sucrose. It is also suggested that stress-induced proteins are involved in the development of desiccation tolerance in plant cells. Water content of M. polymorpha cells decreased to 0.1 g/gDW after desiccation on silica gel for 24 hrs. Survival of cells after desiccation was 0% in the cells without preculture, however, approximately 80% in the preculture with 0.5M sucrose. Expression patterns of heat-stable proteins were changed when cells were precultured in this medium. When cells were precultured in this medium plus 10 μM cycloheximide, survival was approximately 80% after desiccation for 4 hrs, but almost 0% after desiccation for 24 hrs.
These results suggested that de novo synthesized proteins during the preculture participate in fully development of desiccation tolerance in cultured cells of M. polymorpha.
