Abstract
To improve soil environment, utilization of terrestrial cyanobacteria is considered as a practical means. Nevertheless, the mechanism of desiccation tolerance of this organism has not yet been elucidated. We have analyzed the global expression profiles during dehydration in the filamentous cyanobacterium Anabaena sp. PCC 7120. Rehydration process, during which cells recover from damages by the stress, is considered to be important for dehydration tolerance besides enduring during dehydration. We performed DNA microarray analysis during rehydration. Many genes were upregulated during rehydration. Among them, we focused on the ancrpB, whose gene product is a transcriptional factor activated by cAMP. Intracellular cAMP concentration increased transiently when rewetting cells. The recovery of oxygen evolution activity during rehydrartion in ancrpB disruptant was lower than that in wild type, suggesting the importance of the gene regulation by AnCrpB. We will discuss the cAMP signaling during rehydration as well as expression profiles during dehydration and rehydration.
