Abstract
State transition, the redistribution of light-harvesting complex II (LHCII) between photosystem I (PSI) and photosystem II (PSII), is one of the photoacclimation mechanisms in plants and algae. To explore the dynamic changes in the supermolecular organization during state transitions, biochemical isolation of both PSI and PSII supercomplexes including the light-harvesting complexes is demanded. However, there has been as yet no report obtaining the highly pure PSII-LHCII supercomplexes during state transtions. In this study, we establish a new, simple, rapid procedure to isolate highly pure PSII-LHCII supercomplexes from a Chlamydomonas mutant carrying a hexahistidine-tag at C-terminus of CP47. The differences in the size and protein compositions of the supercomplexes between state 1 and state 2 will be reported.
