Abstract
After the completion of the whole genome sequence of model plants, it is now possible functional genomics approach through the comprehensive analyses of metabolites (metabolomics) and gene expression (transcriptomics). We have already integrated metabolomics and transcriptomics of PAP1-overexpressed transgenic plants, two putative glycosyltransferase genes induced by PAP1 expression were confirmed the gene functions, experimentally(1). On the other hand, transcriptome coexpression database (ATTED) was released in last year. In this research, we inferred a coexpression framework model including the putative phenylpropanoid metabolic genes and up-regulated genes by PAP1, and subsequently speculated each gene function. Metabolic profiling of the targeted gene T-DNA insertion mutants were performed using high-throughput analysis based on UPLC-Q-TOF MS. Subsequently, detailed analysis of metabolite changing in the mutants revealed the changes in metabolo-types, indicating the 2 glycosyltransferases and 1 dehydratase gene functions.
1. Tohge et al., Plant J. (2005) 42: 218-235
