Abstract
Plants resistant to Al stress were isolated from Arabidopsis enhancer-tagging lines. RT-PCR experiments indicated that one of the resistant candidates (#355-2) showed a higher expression of two genes on chromosome 1, F9E10.5 and F9E10.6, compared with Col-7 control line. Transgenic plants over-expressing the F9E10.5 gene showed a higher resistance than a control line.
The #355-2 line always showed a lower Al content in whole roots, and the root hair length of this line was approximately 30% of that of Col-7. Both Al influx and associated oxidative stress occurred in root hairs, as well as in root tips of Col-7, however staining was seen only in root-tips of #355-2. Moreover, transgenic plants over-expressing the F9E10.5 gene showed shorter root-hairs than control.
Resistance of #355-2 line may be due to a lower Al uptake from the short root hair derived from an over-expression of F9E10.5 gene caused by activation tag.
