Abstract
Matrix attachment region (MAR) is DNA sequence bound to the nuclear matrix. It is considered that the sequence of MAR is about 1 kb-long and is existed at intervals of 50-100 kb on a chromosome. In this study, to investigate the function of MAR, we identified many candidates of MAR using the genome tiling array which covers the long arm of the Arabidopsis chromosome V. About the probe DNA used for the array, the protoplast prepared from Arabidopsis were dissolved by Triton X-100 and digested genome DNA by nuclease, and then co-precipitated DNA with nuclear matrix by centrifugation were purified. We confirmed the binding ability of candidates of MAR to the nuclear matrix in vitro. We analyzed the 20 kb genomic region ( 4 genes are included ) containing a candidate MAR in detail, three MARs existed. Here, we will report the position relation between these MARs and four genes.
