Abstract
GAs are phytohormons that play an essential role in promoting seed germination. The seed germination of various plants has been shown to require reactive oxygen species (ROS) such as O2- and H2O2. Based on DNA micoarray analysis and germination test, we have reported that H2O2 promoted the seed germination of Arabidopsis and induced genes involved in GA biosynthesis. O2- was generated following seed imbibition.
To dissect further the role of O2- generated during seed germination in Arabidopsis, we examined the effects of DPI, an inhibitor of NADPH oxidase, on O2- generation and the transcript level of genes involved in GA biosynthesis. O2- generation was slightly suppressed by DPI and correlated with gene expression involved in GA biosynthesis. These are likely to suggest that O2- generated during seed germination contributes to the regulation of gene expression of GA biosynthesis and O2- is produced NADPH oxidase.
