Abstract
In higher plants, transcriptional regulation by chromatin remodeling is suggested to be involved in the embryogenesis and transition of vegetative/reproductive phase.
We analyzed the effects of HDAC inhibitors on seedling growth after germination in Arabidopsis. Treatment with HDAC inhibitors suppressed the post-germination growth and resulted in the no-suppression of the expression of some embryogenesis related-genes. These results suggest that histone deacetylation is related to the repression of embryogenesis-related genes in transition from embryonic to post-embryonic phase.
To clarify which kind of HDAC factors is involved in transition to post-embryonic phase, we performed the screening of lines showing high sensitivity to HDAC inhibitor among some HDAC mutants. By this screening, HDA6:RNAi plant was identified as a line showing growth arrest after germination and ectopic expression of LEC1 under low concentration of HDAC inhibitor. This result suggests that HDA6 is involved in suppression of embryogenesis-related genes in transition to post-embryonic growth.
