Expression analysis of Arabidopsis CDKA and CDKB2

Abstract

Cyclin-dependent kinases (CDKs) are key regulators of the cell cycle. Although CDK activities are regulated by phosphorylation and binding of regulatory subunits, such as cyclins, little is known about transcriptional, translational and other post-translational regulations of CDKs.
CDKA (CDKA;1) is a functional homolog of budding yeast CDC28, and its expression is cell cycle phase-independent. CDKB2 is unique to plants and is specifically expressed during G2 and M phase. For expression analysis, promoter::GUS transgenic plants have been generated and analyzed.