Abstract
Plant cytokinesis is accomplished with the formation of phragmoplast, which is formed between two daughter chromatins during anaphase. The development of phragmoplast is shown to be regulated by the MAP kinase cascade. The member of MAP kinase cascade being identified, the targets of the cascade are little known. We applied the phosphoprotein purification with immobilized metal ion affinity chromatography and two dimensional-differential gel electrophoresis for the identification of the phosphorylated targets of MAP kinase cascade in Arabidopsis. Since some parts of proteomes obtained from nack1-1 and mpk4-2 mutants were found similar, it was suggested the MPK4 is one of downstream of MAP kinase cascade from AtNACK1. One of the up-regulated spots in nack1-1 mutant found to be the AtCDC48A, which seemed to raise a new target of MAP kinase cascade.
