Abstract
The amount and activity of PSI/II are known to change depending on the light conditions inside leaves of C3 plant. C4 cells will also adapt to the inner leaf condition. We measured the fluorescence spectrum of each cell in a leaf and stem of Setaria viridis (NADP-ME type C4 plant) by the high-spatial-resolution microspectroscopy at room temperature and 77K. The device has a monochrometor attached to the conforcal laser scanning microscope, and has the spatial resolution of 0.3 μm and the wavelength resolution of 1 nm. We can get in vivo spectrum of every single cell and chloroplast. We measured the fluorescence spectrum at various parts in the leaves and figured out PSI/II ratio. In the mesophyll cells, PSI/II ratio is different from that in the bundle sheath cells. We will discuss the relation of the cell environments and the amounts of PSI and PSII.
