Microarray Analysis Of The Genes Involved In The Enhanced Photosynthesis And Productivity In The Transgenic Plants Having Cyanobacterial FBP/SBPase

Abstract

We have previously demonstrated that transgenic tobacco plants expressing FBP/SBPase in chloroplasts exhibit increased photosynthetic capacity and dry matter compared with those of the wild-type plants. To clarify the genes that are associated with increased parameters using a DNA microarray analysis, we produced transgenic Arabidopsis expressing FBP/SBPase in the chloroplasts (ApFS). In ApFS-2 plants with 3.3-fold higher FBPase activity compared to wild-type plants, both fresh weight and photosynthetic CO₂ fixation rate were 1.7-fold higher than those in the wild-type plants. Total RNAs were isolated from hypocotyls, young rosette and basal blade of mature rosette and were used for the DNA microarray analysis. Small, but significant, differences were observed in the expression of many genes. Fifteen transcripts showing more than 1.5-fold expression in ApFS-2 were confirmed by real-time RT-PCR.