The expression profile of nitric oxide responsible genes in transgenic Arabidopsis constitutively or trangently overexpressing bacterial nitric oxide synthesis gene.

Abstract

Recently, many articles have shown that in plants as well as animals nitric oxide (NO) plays a key role of many important physiological processes (disease resistance, stomata movement, floral transition and so on). However, little is known about molecular mechanisms of NO signaling in plants. We produced transgenic Arabidopsis plants (cDrNOS) constitutively overexpressing nitric oxide synthase (NOS) from Deinococcus radiodurans. In the last annual meeting of JSPP (the 46th) we showed the dwarfphenotype of the cDrNOS plants. Expression levels of about 25,000 genes in rosetta leaves of the cDrNOS plants were analyzed using a microarray analysis. We also made a transgenic plants (iDrNOS) transiently overexpressing DrNOS gene after ethanol treatment. We will show the expression pattern of genes, which indicated remarkably different expression levels between WT and the cDrNOS plant on the microarray analysis, in the iDrNOS plants after ethanol treatment and discuss the functions of these genes.