Abstract
The core circadian clock in cyanobacteria is composed of three proteins, KaiA, KaiB, and KaiC. This clock widely affects transcriptional regulation. In order to reveal the timing system for transcription, we have tried a systematic and high-resolution analysis for temporal profiles of gene expression. We have chosen a strategy using a genome-wide bioluminescent-reporter system for living cells under various conditions that include environmental changes. The 0.6-1.2 kbp upstream regions of predicted ORFs of Synechococcus elongatus PCC 7942 were used for the reporters as [promoters]. As a first step, we intensively analyzed reporter strains for 124 ORFs in a genome region (5% of the whole genome). The environments with various light and temperature conditions were analyzed for them. We also compared bioluminescence traces between wildtype- and a circadian clock-less mutant background cells. Cloning/monitoring strategies and temporal characterization of promoter activities will be discussed.
