Analysis of root-hair mutants of Arabidopsis using Fourier Transform Infrared Microspectroscopy

Abstract

A combined Fourier-Transform Infrared (FTIR) microspectroscopy and principle component analysis (PCA) was used to investigate chemical variations between wild-type and root-hair mutants in Arabidopsis. Using this system, we showed that the gl2 mutation affected cellulose synthesis in roots. There are ten genes encoding cellulose synthase (AtCesA1 to 10) in Arabidopsis, five of them are expressed in the root, and of them, only the AtCesA5 gene was affected by the gl2 mutation.