Abstract
In contrast to animal cell, the fundamental mechanism to establish cell polarity in plant has been less depicted. Recent studies on several proteins raised the model that plant cell polarity is mainly defined by an ununiform accumulation of plasma membrane proteins under dynamic equilibrium between their endocytosis and membrane expression via vesicle trafficking. To verify this hypothesis, plasma-membrane localized SNAREs, which are essential to membrane fusion in vesicle transport, were used as marker proteins of vesicle trafficking. Transgenic plant lines expressing GFP-fused plasma membrane SNAREs under control of their own promoters have been established in A. thaliana and P. patens. In addition to microscopic observation, new insight by phylogenetic analysis of SNARE family between these organisms will be reported.
