Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 47
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Functional analysis of extracellular components using immunomodulation.
*Naoki ShinoharaMiki FujitaHiroo Fukuda
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Pages 675

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Abstract
To investigate phenotypes of a plant that expresses a monoclonal antibody is potentially useful to know in situ functions of its antigen. This method is called immunomodulation. It has been reported that plants expressing monoclonal antibodies against phytohormones show phenotypes similar to those of plants short in the phytohoromones.
We had isolated approximately one hundred of monoclonal antibodies that recognize molecules secreted from differentiating xylem cells by phage display subtraction. In this study, to find extracellular components that have important roles in xylem development, we introduced genes for the isolated antibodies into Arabidopsis plants for immunomodulation. The following three points were devised: 1) a promoter for antibody expression, 2) signals directing intracellular localization of antibodies, 3) negative control for evaluation of the causal linkage between phenotypes and antigen-antibody binding. We will report the results of the experiments.
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© 2006 by The Japanese Society of Plant Physiologists
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