Abstract
In the vesicle transport systems of eukaryotes, COPI (coat protein I) and COPII (coat protein II) work in the transport system between ER and Golgi apparatus. COPII that consists of the small GTP-binding protein, Sar1p and two coat protein complexes, sec23p/24p complex and sec13p/31p complex, work in the transportation from ER to golgi apparatus. In plants, vesicle transport system involves in cell division. In this research, we examine the function of COPII components in development of Arabidopsis thaliana.
In these COPII components, we carry out the analysis of homologs of sec31p, sec13p, sec23p and sec24p that each ATSEC31, ATSEC13, ATSEC23 and ATSEC24. The homology search indicates that there are two homologs for each ATSEC31 and ATSEC13, are seven homologs for ATSEC23 and are three homologs for ATSEC24. In this research, we carry out the expression analysis by promoter GUS and the localization analysis by fluorescence reporters for these homologs.
