Abstract
Plants respond to wounding caused by mechanical stress or chewing of herbivore by induction of a various genes that act on wound healing and protection from subsequent invasion by phytopathogens. We had been reported that tobacco ERF genes were immediate early responsive genes to wounding. In addition, these genes were also induced by a translational inhibitor, cycloheximide (CHX). In this study, we performed macroarray analysis with probe sets for approximately 300 genes including transcription factors (AP2/ERF, Dof and Nac family). Twenty and 12 genes were identified as immediate early wound-responsive genes and CHX-responsive genes, respectively, with a greater than 3-fold change over control. All of overlapped 3 genes were encoding the ERF subfamily. On the other hand, 8 genes that belong to the Nac family were specifically up-regulated by wounding. Moreover, we analyzed in detail the expression pattern of these identified genes by real time PCR.
