Abstract
Laser Microdissection (LMD) allows for the one-step procurement of large homogeneous populations of cells from tissue sections. To date, analyses of global gene expression have generally been conducted on whole organs. Such analyses have the potential to mask genes of interest, that is, those that are specifically expressed in a particular cell type. LMD/microarray analyses will provide a powerful approach to monitor gene expression in some specific cell types of plant mutants.
In this study, we germinated the reduced adh activity (rad) mutant and wild type under submerged conditions. The rad mutant shows that elongation of coleoptiles is repressed under submergence. We used LMD for isolation of coleoptiles of the rad mutant and the wild type, extracted RNA and performed microarray experiments. We identified some genes whose transcript levels were different between the rad mutant and the wild type.
