Abstract
Graminaceous plant secretes mugineic acids phytosiderophore (MAs) from its root to solubilize Fe(III), and then take up the Fe(III)-MAs. The analysis of the maize mutant yellow stripe1 (ys1) revealed an Fe(III)-MAs complex transporter YS1. By searching ZmYS1-like gene (OsYSLs) in rice genome, we identified 18 putative OsYSL.
In this study, we show two OsYSLs are involved in the uptake and translocation of iron. OsYSL15 is expressed in the root epidermis and its protein transports Fe(III)-deoxymugineic acid complex. Therefore, it is conceivable that OsYSL15 functions in Fe uptake in the roots.
OsYSL2 was expressed in the Fe-deficient shoots and its protein transported divalent metal-nicotianamine complex. In addition, OsYSL2 was expressed during reproductive stage. OsYSL2 would function in Fe translocation into seed. Now we analyzing OsYSL2 RNAi plant to examine the Fe translocation during reproductive stage.
