Abstract
Plants are exposed to environmental stressors, and most of them generate reactive oxygen species (ROS). It is known that salicylic acid (SA) and ethylene (ET) act as signal molecules to enhance and induce cell death under the ROS signal. However, in our present studies, the SA deficient (sid2) or ET insensitive (ein2) mutant of Arabidopsis showed slightly sensitive to one of the oxidative stressors, ozone. This result suggested that SA and ET have unknown mechanism for reducing ozone damage. To get further information of protection mechanism by SA and ET, we generated double mutants between the ozone sensitive ascorbate deficient mutant (vtc1) and sid2, ein2. These double mutants showed higher level of leaf injury than that vtc1. This demonstrates that SA and ET have protective aspect for ozone. We are analyzing how SA and ET are involved in protection to ozone using vtc1/sid2, vtc1/ein2 double mutants.