Abstract
For genetic improvement of plural traits in a short time, we tried to develop new breeding method. In this method, firstly, by combination of introduced chromosome region genotyping and phenotyping, superior chromosome region is left and inferior region is replaced. We identified the chromosome region affect various trait including inferior or superior traits (CRATs).
BIL (backcross inbred lines) were randomly substituted 'Koshihikari' chromosome with 'Kasalath' fragment. SNP analysis was used for genotyping. BIL lines were crossed with 'Koshihikari', next self-pollinate, we got BC1F2 progeny. Using the genotyping with results of CRAT analyses, four superior lines were selected among 500 BC1F2 plants and planted to observe their phenotype. We got a superior line, that was same as 'Koshihikari' to heading data and plant height. At a present, we examine the traits, ratio of filled grain, grain number, pushing resistance, et al., we confirm the effect of replacing.
