Abstract
We isolated a dominant mutant, cryptic precocious-1D (crp-1D) which dramatically enhances the precocious-flowering phenotype of 35S::FT. CRP gene was identified by the positional cloning. CRP encodes a large protein which has low similarity to a subunit of the transcriptional mediator complex. To gain insights into its function, we analyzed morphological phenotype of crp-1D mutant in the absence of the 35S::FT transgene. Loss-of-function mutants of crp were obtained from Ds and T-DNA insertion lines and analyzed. crp-1D mutant showed slightly early-flowering phenotype compared with wild type. By contrast, crp loss-of-function mutants showed late-flowering phenotype. These observations suggest that CRP play some role in regulation of flowering. Interestingly, crp loss-of-function mutants also showed pleiotropic phenotype including dwarfism, curled leaf, abnormal shape of floral organs, and sterility. Characterization of the phenotype and analysis of expression patterns will be presented.
