Abstract
Plant peroxisomes play a critical role in many aspects of growth and development from germination to leaf senescence. In the photosynthetic tissues, peroxisomes contain enzymes that participate in photorespiration, and share the metabolic pathway with chloroplasts and mitochondria. There are some reports on investigating the morphology and motility of peroxisome in root and stem cell (Mano et al, 2002), but few report on those in leaf mesophyll cell. Here we observed localization of peroxisome in Arabidopsis leaf mesophyll cell using transgenic plants stably expressing the fluorescence protein that enable to visualize peroxisomes and cytoskeletal proteins. We investigated then the proper condition regulating the peroxisome localization in detail. Furthermore, we screened a number of the peroxisome unusual positioning mutants (peup) from EMS treated mutant lines. The physiological analyses of peroxisome in wild-type and peup lines show leaf peroxisome having specific mechanism in taking their localization in mesophyll cell.
