Abstract
PYK10/BGLU23 is a β-glucosidase that localized in ER bodies. Active PYK10 molecules are involved in a large complex with diameters ranging from 0.65 μm to over 70 μm. Expression levels of some members of jacalin-related lectins (JALs) and GDSL lipase-like proteins (GLLs) are lower in the nai1-1 mutant, which have no ER body, than in Col-0. The analysis of public DNA microarray dataset reveals that some of JALs and GLLs coexpress with PYK10. These results of expression analysis suggest that JALs and GLLs are good candidates of the interacting partners of PYK10. To identify members of the active PYK10 complex, the complex is purified. Some JALs and GLLs are found in the active PYK10 complex. The active PYK10 complex is larger in jal and gll mutants than in Col-0. The difference of subcellular localization of PYK10, JALs and GLLs suggests that their interactions occur when subcellular structure is destroyed.
