Abstract
To identify large-scale useful rice gene, we are established rice FOX lines using 13,000 non-redundant rice full length cDNAs to introduce them into Arabidopsis plants.
UV-B radiation can damage plants due to destruct several cell components and reduce gene expressions. To isolate useful rice genes involved in tolerance to UV-B stress, we try to screen UV-B tolerant mutants from rice FOX lines.
We already isolated 50 UV-B tolerant mutant candidates in T2 generation from ca. 3,900 lines by root bending assay screening. Each isolated mutant contained one or two copies of rice cDNA which may encode cell-cycle related genes, stress responsive genes, transcription factors and some unknown function genes. Now we differentiate the isolated mutants based on phenotypes of root growth with or without UV-B irradiation.
This research is supported by the Special coordination funds for Promoting Science and Technology entitled Rapid identification of useful traits using rice full-length cDNAs.
