Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 48
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Screening of Ultraviolet-B Tolerant Mutants Using the Rice FOX Lines
*Shinya TakahashiTomoko KuriyamaTakanari IchikawaYouichi KondouYukako HasegawaMika KawashimaShu MutoHirohiko HirochikaMinami Matsui
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Pages 095

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Abstract
To identify large-scale useful rice gene, we are established rice FOX lines using 13,000 non-redundant rice full length cDNAs to introduce them into Arabidopsis plants.
UV-B radiation can damage plants due to destruct several cell components and reduce gene expressions. To isolate useful rice genes involved in tolerance to UV-B stress, we try to screen UV-B tolerant mutants from rice FOX lines.
We already isolated 50 UV-B tolerant mutant candidates in T2 generation from ca. 3,900 lines by root bending assay screening. Each isolated mutant contained one or two copies of rice cDNA which may encode cell-cycle related genes, stress responsive genes, transcription factors and some unknown function genes. Now we differentiate the isolated mutants based on phenotypes of root growth with or without UV-B irradiation.
This research is supported by the Special coordination funds for Promoting Science and Technology entitled Rapid identification of useful traits using rice full-length cDNAs.
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© 2007 by The Japanese Society of Plant Physiologists
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