Abstract
Previous studies by Vassileva and colleagues (2005) demonstrated that microtubules slowed transiently in dynamics to the addition of liposaccharides (Nod factors) in living root hairs of transgenic Lotus japonicus cv. Gifu containing the fusion construct of green fluorescent protein and tubulin-α 6.
Our aim is to determine how the microtubule dynamics change during three important events needed for successful root hair infection by rhizobia i.e. during root hair curling, infection thread (IT) development and growth inside the root hair.
At 3 d, 4 d and 5 d post inoculation, GFP-labeled cortical and ER microtubules in root hairs of Mesorhizobium-treated transgenic L. japonicus plants displayed typical dynamics and behaviour patterns during root hair curling, IT development and growth. Furthermore, the behaviour of GFP-labeled microtubules in relation to nucleus positioning was also monitored.
