Abstract
Arabidopsis bZIP transcription factor AtbZIP10 directly interacts with programmed cell death (PCD) regulator LSD1 in cytosol and its function is also involved in the regulation of PCD (1). The interaction with exportin, AtXPO1, would be required for the nuclear export of AtbZIP10 (1). To identify unknown proteins involved in the regulation of AtbZIP10 subcellular localization, we performed Yeast-Two-Hybrid screening and have focused on the microtubule-plus-end-binding protein AtEB1(end-binding 1) from plural positive clones identified by screening. We found that AtbZIP10 interacts with all Arabidopsis EB1(EB1a,b,c) and that the leucine zipper-like region at C-terminal domain of AtEB1s specifically binds to C-terminal domain of AtbZIP10 which does not contain bZIP domain. The function of AtEB1 is not clear yet, but the interaction between AtbZIP10 and AtEB1 suggested that it participates in the control of AtbZIP10 localization in cytosol. (1) Kaminaka et al., EMBO J. (2006)
