Abstract
Plants produce a lot of hydrophobic metabolites including phospholipids, glycolipids. These compounds generally classified into lipids are diverse with their chemical structures and biological functions. To comprehend the chemical profile and biosynthetic network of various plant lipids, we have started the analysis of lipid extract using LC-MS.
Lipid fractions of Arabidopsis thaliana were separated and analyzed using hydrophilic interaction chromatography (HILIC) coupled to Shimadzu ion-trap (IT)-TOF mass spectrometer. Separation was archived by using the mixture of acetonitrile, methanol, and water containing ammonium formate as HPLC solvent. The peaks attributable for typical membrane lipids (phospholipids and glycolipids) and glycosylated terpenoids were determined by chromatographic behavior and their accurate mass. During the optimization of analytical conditions, lipids extracted from different tissue and mutant plants were also analyzed. We would like to report some of the data.
