Abstract
Rice Sekiguchi lesion (sl) mutant spontaneously induces a run-away type cell death on its leaves without an infection of pathogens. Recently, a gene responsible for the mutation has been isolated by map-based cloning and assigned as a putative cytochrome P450 (CYP71P1) based on the homologies with other plant CYPs. In this study, the metabolic profile of sl mutant was analyzed to estimate the function of CYP71P1 gene. The metabolites extracted from three lines of rice suspension cell cultures, wild type (WT), sl mutant (SL), and CYP71P over-expresser under sl mutant background (OX) were served for the metabolic profiling analysis using LC-ESI-MS (Shimadzu LCMS-2010). The analysis of the metabolic profile data indicated that level of tryptamine was increased while that of serotonine was decreased in sl mutant cells. The same phenotype was also observed in the leaf tissue of sl mutant suggesting that the function of CYP71P1 is tryptamine 5-hydroxylase.
