Abstract
Plants release a certain amount of photosynthate as Rhizodeposition. Though individual compounds have been surveyed so far, total of the root exudates is not figured out. We have established aseptic cultivation system of rice to obtain root exudates without microbial contamination. The results of global analyses of root exudates by Gas Chromatography - Mass Spectrometry (GC-MS) are reported.
Root exudates was collected from rice (Oryza sativa cv. Nipponbare) grown under +P / -P conditions on hydroponic culture. Sterility of the system was confirmed by 1 / 10 SCD medium. Root exudates were eluted by methanol from freeze dried sample, and then analyzed by GC-MS. About 90 peaks were detected and a half of which were identified by AMDIS software. Under P deficiency, decreased exudation of sugars and increased amines and fatty acids were indicated by PCA. Further analysis with UPLC-MS, expanded for secondary metabolites is in progress.
