Purple acid phosphatase in the wall of tobacco cells

Abstract

The role of plant purple acid phosphatases (PAPs) has ever been proposed for the degradation of organophosphates, when plants are under phosphate starvation. Nevertheless, phosphate deprivation did not induce some PAPs in tobacco cells. In the transgenic tobacco cells overexpressing the PAP (NtPAP12), not only wall regeneration but also cellulose deposition was accelerated on the surface of tobacco protoplasts. Since PAPs are members of a group of nonspecific phoshomonoesterase, containing protein phosphatases, calcineurin (type 2B) and protein phosphatases type 1, the tobacco PAP could probably function as the type of protein phosphatase. We have identified some potential substrates for NtPAP12 in suspension-cultured tobacco cells by phosphoproteome.