Abstract
RNA editing is a process converting specific C to U on chloroplast mRNA. crr4 mutants are defective in the RNA editing that creates an initiation codon of the chloroplast ndhD gene. CRR4 specifically recognizes and binds to the sequences surrounding this editing site. It is unlikely that CRR4 contains any domain responsible for RNA editing enzyme activity, such as cytidine deaminase, suggesting that CRR4 interacts with an unidentified RNA editing enzyme.
In this study, we have screened for suppressors of crr4-4 to elucidate the RNA editing machinery by determine the factor interacts with CRR4. crr4-4is a weak allele of crr4. We obtained 30 suppressor candidates from 25,000 M2 plants treated with EMS. Although NDH protein level was restored in some lines, the editing phenotype was not drastically suppressed.
