Abstract
The moss Physcomitrella patens development starts from a germinating spore that gives rise to a filamentous structure called protonema. Bud that is arisen form protonema develops to give rise to a leafy shoot called gametohpore. After dissection of a leaf from a gametophore, leaf cells facing to the dissected cells change to protonemal apical stem cells. However, the molecular mechanisms of this process have been unknown. To understand the mechanisms, we focused on the cell cycle regulation during the transdifferentiation after leaf dissection. In this study, we searched the DNA database for P. patens genes encoding core cell-cycle regulators. We next investigated the accumulation pattern of transcripts of the cell-cycle regulators in dissected leaves with real-time RT-PCR analysis. The result revealed that transcripts encoding some cell-cycle regulators increased in dissected leaves. Further experiments are directed to the functional analysis of the cell cycle-related genes during the transdifferentiation.
