Abstract
The unicellular green alga chlamydomonas reinhadtii has no enzyme to convert a single strand RNA into a double strand. This leads to that continuous supply of large amount of double strand RNA is required to achieve stable RNAi. However, we have been observed that inverted repeat DNA construct drove by the rbcS2 promoter, which is one of the most effective polII promoter, can induce a transient RNAi. We noticed the report that silence DNA construct drove by tRNA promoter is very successful in human cell. So far, we made six kinds silencer DNA constructs bearing modification at the acceptor stem of C.reinhardtii Asp tRNA, which is targeted the aadA mRNA conferring spectinomycin resistance. Preliminary experiment showed that some of them have much bigger efficiency than the polIII promoter drove type silence construct.
