Abstract
Soybean is important crop since soybean seeds contain a large amount of protein and lipid, and useful as functional foods and energy source. However transformation efficiency of soybean is lower compared to rice and tobacco. Therefore, the technology of transgenic soybean was not so common. Transformation using Agrobacterium tumefaciens has advantage to introduce the low copy transgene stability. We aim to establish the soybean transformation using Cotyledon node method with A.tumefaciens to produce the transgenic soybean containing high amount of nicotianamine (NA) in their seeds. NA has the ability to suppress high pressure. We have established the condition to get the regenerates after many trial and errors. We are improving transformation efficiency more by examination the condition as germination status, hygromycin concentration, light intensity and temperature.
