Abstract
We were establishing lines for genome wide functional analysis of Arabidopsis genes by employing gain-of-function techniques, activation tagging and FOX hunting system. However the isolation of important genes, that cause severe phenotypes like embryonic lethal, is difficult from these lines for constitutive 35S promoter. And many transcription factors should be included in these important genes, because they play pivotal role for regulation of various biological functions in plants. We are, therefore, establishing novel lines, and the feature of these lines is the functional induction of transcription factors transformed in plants by employing glucocorticoid-mediated transcriptional induction system. And the important knowledge might be acquired by comparison between these lines and CRES-T lines, that are established by employing gene silencing system using dominant repressor, since they shows loss-of-function and gain-of-function phenotypes, respectably. In this report, we describe about the detail of these lines and discuss the usefulness as genome resource.
