Abstract
Fluorescence Lifetime Imaging Microscopy (FLIM) is a technique to visualize the fluorescence lifetime in cells. The fluorescence lifetime is the time of fluorophore to remain at the excitation state before returning to the ground state. FLIM can be used to measure FRET, pH, ion concentration, amount of reactive oxygen species, etc. I applied FLIM to analyze the organellar environment. Various GFP lines targeted to the organelles were used to examine the difference in the lifetime depending on the organelles. In addition, the effect of pH change over the lifetime was examined. These data will be a basis of future research on the intracellular dynamics during development or under various environmental stresses.
