Abstract
We have been working on phytoremediation, which is a method to clean soil and water of pollutants such as heavy metals using plants. Many researches have been carried out to enhance the ability of tolerance and accumulation of heavy metals by a gene manipulation technique, however, few have succeeded. We employed T-DNA tagged mutants to understand the mechanism of the heavy metal tolerance and accumulation ability of plants. In this study, to identify heavy metals tolerance determinants, we screened approximately 40 hyper sensitive mutants of cadmium (Cd) from a T-DNA tagged population which involved more than 13000 lines in Arabidopsis Col-0 gl-1 background. These mutants showed higher sensitivity to Cd than wild type under the condition of 50 μM Cd. TAIL-PCR revealed the location of the introduced T-DNA in mutant genome.
