Abstract
Plant cell walls are dynamic structures that involved in the morphology, growth, and development of cells and tissues. We produced the mutants that were abnormal in intercellular attachment by activation tagging. We introduced pPCVICEn4HPT vector into Nicotiana tabacum and obtained seven mutant lines that had defective and fragile organs. We named them shoolac (shoot with loosely attached cells). For investigation of variation of shoolac1 we isolated cell walls, fractionated them to pectin and hemicellulose fractions and subjected each fraction to sugar composition analysis. The arabinose contents of 4M KOH-soluble hemicellulosic fractions of shoolac1 were higher than that of wild one, 4M KOH-soluble hemicellulosic fractions were digested with xyloglucan-specific endoglucanase and the released xyloglucan oligosaccharides were analyzed by LC-MS and 1H NMR spectroscopy. Shoolac1 xyloglucan had almost two times higher arabinose content than the wild type. This result indicates that increase in arabinose content in xyloglucan affects cell adhesion.
