Abstract
Most Chl a in the PS1RC complexes was removed without any loss of P700 by ether treatment, yielding the antenna-depleted PS1RC complexes that contain 12-14 Chl a per P700. (1) On addition of Phe a, Zn-Chl a and Cu-Chl a with phospholipids (PG), about one-third of the added Zn-Chl a was bound, similarly with Chl a, to the complexes. Phe a bound a half less than Chl a. Cu-Chl a bound exponentially with increasing its amounts added. (2) The initial rate of the P700 photooxidation increased by the number of the Zn-Chl a bound, while it was not enhanced by the bound Phe a or the bound Cu-Chl a. (3) These results suggest that the bound Zn-Chl a acts as efficient antenna as Chl a, while Phe a and Cu-Chl a could not bind to the Chl a-binding sites in the complexes, resulting in no recovery of antenna activities.
