Abstract
We first set up on the experiment system with tobacco suspension cultured cells, of which Hypersensitive Response (HR) can be induced by the TMV avirulence factor p50 upon dexamethasone (DEX) treatment. This system makes it available to synchronously induce HR, by which sets of relevant genes are easily screened. A transcription profiling with an oligonucleotide array representing about 10,000 Nicotiana tabacum genes, identified over 400 genes that were up regulated at 10 h after DEX treatment. The numbers of down regulated genes were 300. Depending on these results, we classified genes into two major groups, one those directly controlled by the N factor, and the other those participating in general defense response. In addition, there is the signal transduction pathway that does not mediate genes. Therefore we performed a search of the factors that interacted with N factor by yeast two-hybrid method. As a result, three candidates were provided.
