Abstract
Apyrase is a low substrate spesificity enzyme that hydrolize NTP and NDP. Apy1 and Apy2 are paralogues of apyrase in pea. In our study we found that APY1 is present more in the cytoskeletal fraction obtained from first internode of dark-grown pea. In order to find the role of apyrase in germination and differentiation, we analyzed the expression of apyrase using western blotting, RT-PCR and immunohistochemistry. The expression of APY1 appeared suddenly after 10 h of germination in all tissues studied and it reached a maximum in 62 h in root, stem and leaf tissues. Our reference genes (ACT and TUB) were induced during imbibition and germination but, we found that APY1 is the induced only during germination and not during imbibition. And in the later stages a tissue specific expression of Apy1 was observed. These results suggest that APY1 plays an important role in germination and differentiation in pea.
